9/20/2023 0 Comments Cytoscape gene expressionThe analysis of enriched KEGG pathways revealed the association of the DEGs in ubiquitin-mediated proteolysis and cardiac muscle contraction. The GO analysis of DEGs with significant differences revealed that they are involved in critical biological processes and molecular pathways, such as myeloid cell differentiation, peptidyl-lysine modification, signaling pathway of MyD88-dependent Toll-like receptor, and cell-cell adhesion. Results: We investigated the top 250 significant DEGs ( p-value < 0.05 fold two change ≥ 1 or ≤ −1). The identified DEGs were subjected to the DAVID v6.8 webserver and ClueGo/CluePedia for functional annotation, such as gene ontology (GO) and enriched molecular pathway analysis of DEGs. MCODE was used to determine the gene cluster in the interactive networks. The interaction between the DEGs was identified by applying the STRING tool and visualized using Cytoscape software. Methods: The Affymetrix microarray dataset (GSE13985) from the GEO database and the GEO2R statistical tool were used to identify the differentially expressed genes (DEGs) from the white blood cells (WBCs) of five heterozygous FH patients and five healthy controls. This study focused on identifying the dysregulated molecular pathways and core genes that are differentially regulated in FH and to identify the possible genetic factors and potential underlying mechanisms that increase the risk to atherosclerosis in patients with FH. 3Department of Biomedical Sciences, College of Health and Sciences, Qatar University, QU Health, Doha, Qatarīackground and Aims: Familial hypercholesterolemia (FH) is one of the major risk factor for the progression of atherosclerosis and coronary artery disease.2Department of Biotechnology, College of Biomedical Sciences Technology and Research, Sri Ramachandra Institute of Higher Education and Research (DU), Chennai, India.1School of Biosciences and Technology, Vellore Institute of Technology, Vellore, India.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |